Journal: British Journal of Nutrition

Article Title: Effect of β-carotene-rich tomato lycopene β-cyclase (tlcy-b) on cell growth inhibition in HT-29 colon adenocarcinoma cells

doi: 10.1017/s0007114508169902

Figure Lengend Snippet: Fig. 1. Effects of tomato lycopene b-cyclase (tcly-b) tomato digestate on the growth of HT-29 cells. (A) Effects of different digestate concentrations for 24 h: (A), control; (B), digestate at 20 ml/l; (o), digestate at 50 ml/l; ( ), digestate at 100 ml/l. Values are the means of three different experiments, with standard errors represented by vertical bars. a,b,c Mean values with unlike letters were significantly different (P,0·05; Fischer’s test). (B) Effects of different times (24, 48, 72 h) of treatment: (A), control; ( ), digestate at 100 ml/l. Values are the means of three different experiments, with standard errors represented by vertical bars. a,b,c,d Mean values with unlike letters were significantly different (P,0·002; Tukey’s test). The treatment–time interaction was significant (P,0·05).

Article Snippet: HT-29 human colon adenocarcinoma cells (American Type Culture Collection (ATCC), Rockville, MD, USA) were grown in minimum essential medium.

Techniques: Control

Journal: British Journal of Nutrition

Article Title: Effect of β-carotene-rich tomato lycopene β-cyclase (tlcy-b) on cell growth inhibition in HT-29 colon adenocarcinoma cells

doi: 10.1017/s0007114508169902

Figure Lengend Snippet: Fig. 2. Effects of varying tomato lycopene b-cyclase (tcly-b) tomato digestate concentration on apoptosis induction in HT-29 cells: (A), control; (B), diges- tate at 20 ml/l; (o), digestate at 50 ml/l; ( ), digestate at 100 ml/l. (A) Percen- tage of terminal deoxynucleotidyl transferase-mediated dUTP nick end labelling (TUNEL)-positive cells. (B) Caspase-3 activation. Cells were treated for 24 h. Values are the means of three different experiments, with standard errors represented by vertical bars. a,b,c Mean values with unlike letters were significantly different (P,0·05; Fischer’s test).

Article Snippet: HT-29 human colon adenocarcinoma cells (American Type Culture Collection (ATCC), Rockville, MD, USA) were grown in minimum essential medium.

Techniques: Concentration Assay, Control, TUNEL Assay, Activation Assay

Journal: British Journal of Nutrition

Article Title: Effect of β-carotene-rich tomato lycopene β-cyclase (tlcy-b) on cell growth inhibition in HT-29 colon adenocarcinoma cells

doi: 10.1017/s0007114508169902

Figure Lengend Snippet: Fig. 3. Bax, Bcl-2 and Bcl-xl expression in HT-29 cells treated with varying tomato lycopene b-cyclase(tcly-b) tomato digestate concentrations for 24 h: (A), control (C); (B), digestate at 20 ml/l (T20); (o), digestate at 50 ml/l (T50); ( ), digestate at 100 ml/l (T100). (A) Representative Western blot analyses. (B) Bcl-2 densitometric analysis. (C) Bcl-xl densitometric analysis. Values are the means of three different determinations, with standard errors represented by vertical bars. a,b,c Mean values with unlike letters were significantly different (P,0·05; Fischer’s test).

Article Snippet: HT-29 human colon adenocarcinoma cells (American Type Culture Collection (ATCC), Rockville, MD, USA) were grown in minimum essential medium.

Techniques: Expressing, Control, Western Blot

Journal: British Journal of Nutrition

Article Title: Effect of β-carotene-rich tomato lycopene β-cyclase (tlcy-b) on cell growth inhibition in HT-29 colon adenocarcinoma cells

doi: 10.1017/s0007114508169902

Figure Lengend Snippet: Fig. 6. b-Carotene incorporation and/or association in HT-29 cells treated with tomato lycopene b-cyclase (tcly-b) tomato digestate (B) and purified b-carotene (X) for 24 h. The concentration of digestate in cultured medium was 100 ml/l and that of the purified carotenoid was 0·8 mM. Values are the means of three different experiments, with standard errors represented by vertical bars. * Mean value was significantly different from that of the digestate-treated cells (P,0·005; Tukey’s test).

Article Snippet: HT-29 human colon adenocarcinoma cells (American Type Culture Collection (ATCC), Rockville, MD, USA) were grown in minimum essential medium.

Techniques: Concentration Assay, Cell Culture

Journal: British Journal of Nutrition

Article Title: Effect of β-carotene-rich tomato lycopene β-cyclase (tlcy-b) on cell growth inhibition in HT-29 colon adenocarcinoma cells

doi: 10.1017/s0007114508169902

Figure Lengend Snippet: Fig. 5. Comparison of b-carotene (X) and tomato lycopene b-cyclase (tcly-b) tomato digestate (B) ability in inhibiting HT-29 cells growth after 24 h treat- ment. Concentrations of tcly-b tomato digestate of 20, 50 and 100 ml/l corre- spond to b-carotene concentrations of 0·08, 0·16 and 0·8 mM, respectively.

Article Snippet: HT-29 human colon adenocarcinoma cells (American Type Culture Collection (ATCC), Rockville, MD, USA) were grown in minimum essential medium.

Techniques: Comparison

Journal: British Journal of Nutrition

Article Title: Effect of β-carotene-rich tomato lycopene β-cyclase (tlcy-b) on cell growth inhibition in HT-29 colon adenocarcinoma cells

doi: 10.1017/s0007114508169902

Figure Lengend Snippet: Fig. 4. Effect of b-carotene on cell growth (A), caspase-3 activation (B), Bcl-2 (C) and Bcl-xl (D) content in HT-29 cells following a 24 h treatment. Values are the means of three different experiments, with standard errors represented by vertical bars. a,b,c Mean values with unlike letters were significantly different (P,0·05; Fischer’s test).

Article Snippet: HT-29 human colon adenocarcinoma cells (American Type Culture Collection (ATCC), Rockville, MD, USA) were grown in minimum essential medium.

Techniques: Activation Assay

Journal: Saudi Journal of Biological Sciences

Article Title: Antioxidant, anticancer, apoptosis properties and chemical composition of black truffle Terfezia claveryi

doi: 10.1016/j.sjbs.2016.01.031

Figure Lengend Snippet: Cytotoxic activities of T. claveryi extracts.

Article Snippet: Human brain carcinoma cell line U-87 MG (HTB-14); human colorectal carcinoma cell line HT29 (CCL-247) and human hormone sensitive and invasive breast cancer cell line MCF-7 (HTB-22) were purchased from ScienCell USA.

Techniques: Inhibition